Human Interphase Lymphocyte Dna Condensation Employing Mitotic Extracts

Premature Chromosome Condensation (PCC) has been shown to be a powerful cytogenetic and cytokinetic tool in understanding cell-cycle analysis(1) and also for diagnostic purposes(2,3). Although PCC is a useful cytogenetic tool, it involves somatic cell hybridizations employing a mitotic partner. Understanding of the factors responsible for chromatin condensation and their mechanism of action can throw better light on the cell cycle kinetics and also the protein repertoire of the cells in the various phases of the cell cycle. A number of proteins involved at various levels of cell cycle have been characterized and a variety of protein kinases have been separated and identified in extracts from mitotic and interphase cells. It is also understood that protein phosphorylation / dephosphorylation may be integral to the mechanism of chromosome condensation(4). The induction of PCC in cells using chemicals was well demonstrated and some of the chemical agents employed ABSTRACT